The Arabidopsis abscisic acid (ABA)–insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes.

Dec 16, 2016 Arabidopsis insertional mutant abi5 was characterized as ABA insensitive in comparison to the wild type during seed germination. The mutation

Här visar författarna att NF-YC-transkriptionsfaktorer kan interagera med RGL2 DELLA-proteinet för att reglera expression av ABI5 och därför modulera ABA- och GA-responsivt genuttryck. Nodes: Network nodes represent proteins ABI5 | Abscisic acid insensitive 5 (peptide antibody) Product information Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana ABI5 sequence,UniProt: Q9SJN0, TAIR: AT2G36270 Host Rabbit Clonality Polyclonal Purity Affinity purified serum in PBS, pH 7.4 Format Lyophilized Quantity 100 µg ABI5 and AREBs/ABFs are considered to have a redundant function and similar binding properties. ABI5 is able to form homo- or heterodimers with other ABF/AREB proteins. For instance, ABI5 was shown to interact with ABF3 and ABF1 (Kim et al., 2002; Finkelstein et al., 2005). 1994-10-01 · Interacts (via C-terminus) with SPK1, SCAR3, ABI5, APRR1, AIP2, AIP3 and AIP4.

S2a,b). Transgenic lines Col‐0 35S:ABI5‐MYC and bes1 Super:ABI5… Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. 2016-09-14 The functional relationship between AtU2AF65b and ABI5 was further tested by using the abi5 atu2af65b‐1 double mutant. The flowering time of abi5 atu2af65b‐1 plants was similar to those of abi5 and atu2af65b single mutant plants (Fig.

This entry also includes ABI5 from Arabidopsis.

2008-12-16 · Mediates E2-dependent protein ubiquitination. Acts as a negative regulator of abscisic acid signaling. Required for ABI5 degradation, by mediating its ubiquitination. Together with EDR1, may regulate endocytic trafficking and/or the formation of signaling complexes on trans-Golgi network (TGN)/ early endosome (EE) vesicles during stress responses.

It is expressed in seeds and up-regulated in response to ABA. Loss of function mutants show decreased rate of germination in the presence of ABA. ABA dependent regulation is mediated by both ABI3 and ABI5. 2008-12-16 · Mediates E2-dependent protein ubiquitination.

2008-12-16

The dgat1 mutant (CS3861) was purchased from TAIR. An abi4‐1 mutant line in a Col‐0 background and an abi5‐1 mutant line in a Ws background were obtained from Dr. Dapeng Zhang.

Transgenic plant generation TheABI4-1345to0andABI5-1097to0promotersequenceswere TAIR Curator Summary Encodes a protein that localizes to the nuclear periphery and affects nuclear morphology. Member of a small gene family in Arabidopsis containing 4 proteins (LNC1-4 or CRWN 1-4) with redundant functions in protection from oxidative damage, control of nuclear morphology and degradation of ABI5. TAIR Curator Summary: Encodes a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. TAIR Curator Summary Encodes a nuclear coiled-coil protein related to the carrot peripheral nuclear protein NMCP1 that is involved in the determination of plant nuclear structure.
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Required for ABI5 degradation, by mediating its ubiquitination. Together with EDR1, may regulate endocytic trafficking and/or the formation of signaling complexes on trans-Golgi network (TGN)/ early endosome (EE) vesicles during stress responses.

To test whether ABT affects early developmental processes through ABI5, we performed western blot analysis to assess ABI5 protein levels in imbibed abt-1D, abt-2, and WT seeds treated with ABA TAIR: AT1G45249: UniProt: Q9M7Q4: Position: 1. AA seq: 416 aa AA seq DB search AT2G36270 (ABI5) 09180 Brite Hierarchies 09182 Protein families: genetic RNA splicing is a fundamental mechanism contributing to the definition of the cellular protein population in any given environmental condition.
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abi5 Encodes a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes.

The wild type and mutant recombinant proteins were produced in E. coli and were assayed for DNA-binding activity on their target promoters by electrophoretic mobility shift assay (EMSA). 2019-02-01 Transgenic lines with similar amounts of ABI5 (En‐2 Super:ABI5‐MYC vs bes1‐D Super:ABI5‐MYC) were selected as a pair, and two independent pairs were selected and used for the following studies (Fig. S2a,b). Transgenic lines Col‐0 35S:ABI5‐MYC and bes1 Super:ABI5… Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters.

Nodes: Network nodes represent proteins

2.2. Transgenic plant generation TheABI4-1345to0andABI5-1097to0promotersequenceswere Therefore, the expression of ABI5, MYB30 and their target genes were evaluated in asp1 and wild‐type seedlings. Expression of ABI5 and ABI5‐target genes, RD29A, RD29B, RAB18, Em6, and ADH1, were induced by exogenous ABA treatment, but the induction was less abundant in asp1 than in wild‐type seedlings (Figure 3A). ABI5-BINDING PROTEIN2 (AFP2) negatively regulates the abscisic acid signal by accelerating ABI5 degradation during seed germination in Arabidopsis (Arabidopsis thaliana). The abscisic acid signal is reported to delay flowering by up-regulating Flowering Locus C expression, but the role of AFP2 in regulating flowering time is unknown. TAIR Curator Summary Encodes a nuclear coiled-coil protein related to the carrot peripheral nuclear protein NMCP1 that is involved in the determination of plant nuclear structure.

Transgenic lines Col‐0 35S:ABI5‐MYC and bes1 Super:ABI5‐MYC were selected similarly (Fig. S2c,d). 2021-03-06 · Encodes a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. Mediates E2-dependent protein ubiquitination.